GC00033

Accession No

GC00033

Strain Name

SD.Nes(tm)-GC/ILAS

Status

Freezed embryo

Gene/full name

Nes / nestin

Gene ID

25491

Background

Sprague Dawley

Type

Knockout

Donor

Lianfeng Zhang, Yuanwu Ma, Xu Zhang, Wei Chen

Disease & Phenotype

Construction Description

Genotyping Protocols

Wt 1218bp，ko510bp

Nestin-ko-F  GTGTCTGGCTGTATCTCAAGATTCTC  

Nestin-ko-R  GTCTCTTGCCATCCTAAGTACTTCCT  

Mating System

Heterozygote x Heterozygote

Reference

1. Ma Y , Ma J , Zhang X. Generation of eGFP and Cre knockin rats by CRISPR/Cas9, Febs Journal. 2015.