GC00001

Accession No

GC00001

Strain Name

SD.Lep(tm)-GC/ILAS

Status

Freezed embryo

Gene/full name

Lep/ leptin

Gene ID

25608

Background

Sprague Dawley

Type

Knockout

Donor

Lianfeng Zhang, Yuanwu Ma,Feifei Guan,Li Zhang, Xu Zhang, Wei Chen

Disease & Phenotype

Metabolic disease

Construction Description

Genotyping Protocols

敲除检测

Leptin-9-Mut-S  GTCCTGGAAGCCTCGCTCTGA 

Leptin-9-Mut-A  TGTGCAGATGTAGCCTTCATGGTG

野生检测

Leptin-9-WT-S    5’GGAAGCCTCGCTCTACTCCACA     

Leptin-9-WT-A    5’TGTGCAGATGTAGCCTTCATGGTG

Mating System

Heterozygote x Heterozygote;

Homozygote is infertile

Reference

1.关菲菲，张旭，陈炜. 利用TALENs技术制备瘦素基因敲除的大鼠,《中国比较医学杂志》, 2013.

2.张丽，关菲菲，张旭. 瘦素基因敲除肥胖大鼠的血糖及病理长期观察,《中国比较医学杂志》, 2014