GC00202

GC00202

SD.MT-Trn E(G14098A)-GC/ILAS

Accession No

GC00202

Strain Name

SD.MT-Trn E(G14098A)-GC/ILAS

Status

Frozen Embryo

Gene/full name  

MT-TRNE

Gene ID  

170619

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen， Lianfeng Zhang

Disease & Phenotype  

mitochondrial myopathy

Construction Description

Genotyping Protocols

Mutation detection primer：

F：TCAAGTCTCCGGGTACTCCT

R：AAATATTGAGGCGCCGTTGG

Standard PCR

PCR条件：94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant rat（♀）cross with wildtype SD rat（♂）;

Maternal inherited

Reference

Qi, X., Chen, X., Guo, J. et al. Precision modeling of mitochondrial disease in rats via DdCBE-mediated mtDNA editing. Cell Discov 7, 95 (2021).