GC00012

Accession No

GC00012

Strain Name

SD.B2m(tm)-GC/ILAS

Status

Freezed embryo

Gene/full name

B2m / beta-2 microglobulin

Gene ID

24223

Background

Sprague Dawley

Type

Knockout

Donor

Lianfeng Zhang, Yuanwu Ma, Xu Zhang, Wei Chen

Disease & Phenotype

immune system diseases

Construction Description

Genotyping Protocols

B2M-F  AGAAGACAGTGCTAAACGCCAGG

B2M-R  TCGCTAACAGTCTCAGAATGCAG

KO 347BP  WT 607BP

Mating System

Heterozygote x Heterozygote;

SPF (specific pathogen-free) conditions are recommended.

Reference

1. Yuanwu Ma,Bin Shen,Xu Zhang. Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9, PLoS One. 2014; 9(3): e89413..