GC00214

GC00214

SD.MT-Trn F(G007A)/MT-Trn V(G1030A)/MT-Trn L2(G11714/5A)-GC/ILAS

Accession No

GC00214

Strain Name

SD.MT-Trn F(G007A)/MT-Trn V(G1030A)/MT-Trn L2(G11714/5A)-GC/ILAS

Status

Live

Gene/full name  

MT-Trn F; MT-Trn V; MT-Trn L2

Gene ID  

170599; 170601; 359842

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen， Lianfeng Zhang

Disease & Phenotype  

mitochondrial myopathy

Construction Description

Genotyping Protocols

突变检测引物：

G007-F：AGGCATCTGGTTCTTACTTCAGG

G007-R：TTATTAAGGTTTAGGGCTAAGCATAGTGG 

G1030-F：GCGGTACTTTATATCCATCTAGAGG

G1030-R：GTTTTTAGGTAGCTCGTTTGGTTTC

G11714/5-F：TGAATCTAACAACAGGAAATCAAATTC

G11714/5-R：GATATAATTCCTACTCCTTCTCATCCAAT

WT     Mut

Standard PCR

PCR条件：94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant rat（♀）cross with wildtype SD rat（♂）;

Maternal inherited

Reference