GC00201

GC00201​

SD.MT-Trn K(G7755A)-GC/ILAS

Accession No

GC00201

Strain Name

SD.MT-Trn K(G7755A)-GC/ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn K

Gene ID  

170615

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen， Lianfeng Zhang

Disease & Phenotype  

MERRF, cardiomyopathy and Leigh Syndrome

Construction Description

Genotyping Protocols

Mutation Detection Primer：

F：TGCTCTGAAATTTGCGGCTC

R：AGTCGGTTGCTGATTAGGCG

Standard PCR

PCR条件：94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant rat（♀）cross with wildtype SD rat（♂）;

Maternal inherited

Reference

Qi, X., Chen, X., Guo, J. et al. Precision modeling of mitochondrial disease in rats via DdCBE-mediated mtDNA editing. Cell Discov 7, 95 (2021).