GC00212

GC00212

SD.MT-Trn L2(G11714/5A)-GC/ILAS

Accession No

GC00212

Strain Name

SD.MT-Trn L2(G11714/5A)-GC/ILAS

Status

Live

Gene/full name  

MT-Trn L2

Gene ID  

359842

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen， Lianfeng Zhang

Disease & Phenotype  

mitochondrial myopathy

Construction Description

Genotyping Protocols

突变检测引物：

F：TGAATCTAACAACAGGAAATCAAATTC

R：GATATAATTCCTACTCCTTCTCATCCAAT

WT   G11714/5A

Standard PCR

PCR条件：94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant rat（♀）cross with wildtype SD rat（♂）;

Maternal inherited

Reference