GC00213

GC00213

SD.MT-Trn V(G1030A/G1032A)-GC/ILAS

Accession No

GC00213

Strain Name

SD.MT-Trn V(G1030A/G1032A)-GC/ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn V

Gene ID  

170601

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen， Lianfeng Zhang

Disease & Phenotype  

Mitochondrial Disease

Construction Description

Genotyping Protocols

突变检测引物：

F：GCGGTACTTTATATCCATCTAGAGG 

R：GTTTTTAGGTAGCTCGTTTGGTTTC

WT           G1030A-G1032A

Standard PCR

PCR条件：94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant rat（♀）cross with wildtype SD rat（♂）;

Maternal inherited

Reference